New intron predictions that failed to splice


(tested using PCR primers specified in the Primers Table.)
ORF
NAME/FUNCTION
5' SPLICE SITE
BRANCH POINT
3' SPLICE SITE
BASE PAIRS
YBR057C
MUM2
GUAUGU
UAUUAAC
CAG
173
YDR318W
MCM21
GUAUGU
CACUAAC
AAG
111
YEL003W
GIM4
GUAUGU,GUAAGU,GUAUGC
UACUAAC
UAG
YHR039C
UNKNOWN
GUAUGU
CACUAAC
CAG
YHR124W
NDT80
GUAUGA
UACUAAC
AAG
<247
YKL133C
UNKNOWN
GUAUGU
CACUAAC
AAG
YML031W
NDC1
GUAUGC,GUAUGU
CACUAAC
CAG
340,263
YNL049C
UNKNOWN
GUAUGC
GGCUAAC
CAG,UAG
YOR180
ECI2
GUAUGA
GACUAAC
AAG
YOR195
SLK9
GUAUGA
UACUAAC
CAG
459
YPL208
unknown
GUACGU,GUACGU
UACUAAC
CAG

 

The above genes were identified by Carrie Davis as containing intron-like sequences.  Tests of intron splicing by RT-PCR were negative for these genes using the primer pairs indicated in the Primers Table.

















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