FastQCFastQC Report
Fri 18 Jan 2019
1A_S50_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename1A_S50_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42543286
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATGGGTCGTATGCACAGTGCCGGTAAAGGTATTTCTTCTTCTGCTATT32081207.540837348577164No Hit
GCTGACAAGTCATACATTGAAGGTACTGCTGTTTCTCAAGCTGACGTCAC19812594.657042711745397No Hit
CCACTGAATTAACCGTTCAATCCGAGAGAGCTTTCCAAAAGCAACCACAC18138204.263469446154207No Hit
CAAGTCTTTTAAGGAAAAACAGTGCGGAAAAATGGCTCACTTTAAAGAAT17820254.188733799265059No Hit
GAAAGCAACAAGACAACTAAGACTAAGCAACAATGCCAAGAGCTCCAAGA13808183.2456778256385745No Hit
GTTGGCTGCTAACACTCACTTAGGTGCTAGAAACGTTCAAGTTCACCAAG13351513.138335388573417No Hit
CAATTAATCAATATACGCAGAGATGGCCCCAGTTAAATCCCAAGAATCTA10964482.5772527303133095No Hit
GCTATTCTAAGTGCCTCCGCTCTAGCTGCTAACTCAACTACTTCTATTCC10216322.4013941941391175No Hit
GTCAAGATCTCGAGACTAGCAATAACAAAATGGGTAGAGTTAGAACCAAG10047972.361822732733903No Hit
CGGTGCTCAAGGTACTAAGTTTAGAATCTCATTAGGTCTACCAGTCGGTG9358662.1997971665846405No Hit
AAGAAACCATTAGATCAATAAGCAATGGGTAGAGTTATTCGTAACCAAAG8582322.017314788519157No Hit
AGGGACATTAAGCAGGATGCTGTAAGAGTATGTTAATACGTGAAATGAGA8201491.927798901100399No Hit
AAAACACACCAGATAATTAGTGCATATATATTAGATCAATATGGTTTTAG8105951.9053417735527058No Hit
GCTATTCTAAGTGCCTCCGCTCTAGCTGGTATGTACACATTCTCCTTTTT4800511.128382513753169No Hit
CAATAACAATTAAGAATGGCTAACGGTACGTGAAAGGGGTGATATCCTGT3746770.8806959575242965No Hit
AAATGGGTCGTATGCACAGTGCCGTATGTTTATTAACACCATAGCGAGAT3527460.829146107801828No Hit
CATGTCAGACTACGAGGAGGCGTTTAACGACGGTAATGAGAACTTCGAAG3371870.7925739445702431No Hit
CCGTAGAATAGGTACAGTGAGACAGTATATTCGAAATGGCCGGTGTTAAA3121610.7337491513937122No Hit
CGATAAAGAACCAAATAGGACTAAAAATGGCCGGCGTTAAAGCTTACGAA3091290.726622292410605No Hit
ATTTACTGAATTAACAATGGATTCTGAGGTTGCTGCTTTGGTTATTGATA2978360.7000775633551203No Hit
CCGCTGGTGCTAATGTCGACAACGTCTGGGCTGATGTTTACGCTAAGGCT2809270.6603321614602126No Hit
AGCTGTTCAGGTTAGATGCAGGCTACCAACAATACGACTGGGGTAAAATC2261590.5315973947099432No Hit
TCCATGCCAGAAGGAGGCCTGCGCCATCCAAGGTTTGTAATATTAACTTC2077140.4882415523803215No Hit
GTTGCTACTGATGTGGCCGCCAGAGGTATCGATGTCAAAGGTATCAATTA1921090.4515612639794679No Hit
CAGCATCTCATAATATGTCTGCAACTGCTAAACACGACAGTAACGCTAGC1811950.42590739229687147No Hit
GCCCTAAAACACAATGGTTACACAACAAGAAAAGGAATTTTTAGAATCCT1504490.353637469376484No Hit
CAATAACAATTAAGAATGGCTAACGACCTTGTTCAAGCTCGCGATAACTC1500790.3527677669280177No Hit
CCACTGAATTAACCGTTCAATCCGAGAGAGCTTTCCAAAAGGTATGAATT1494790.35135743863320756No Hit
GAAAACTAATACAGCAACAGAAATACAAAAGTATACAACATGCCAAGTAC1343780.31586182599999446No Hit
CAAGTCTTTTAAGGAAAAACAGTGCGGAAAAAGTGAGTAAATACCTACTA1308330.3075291363248246No Hit
CGTATTGCTAAAGAACTAAGTGATCTAGAAAGAGATCCACCTACTTCATG1269550.29841371444603504No Hit
CGAACCCATTTCTTTGTCCACCACTTTCAAACAATCTTCTCCAGCTAACC1166980.27430415224625576No Hit
GTTGGGATATTTTTGGTTGGTTCAGAGATGTGTTGGCTTCCCTTGGTCTG1137140.2672901195267333No Hit
GAAAGCAACAAGACAACTAAGACTAAGCAACAATGCCAAGTACGTATTAG1127020.2649113658028202No Hit
GCCCGACCTTTGTGTTTCGTACAAGAAAATTGCGCCACCAAAAGGACTAT1118260.26285228649239745No Hit
TGAAAAAGGGCGAAGTCAGTTTAGGTACCTCAATTATGGCCGTGACATTT1058230.248741951902822No Hit
TTTCTCTGGCTTCCCAGTTACTGAAGACGGTAAGTGTCCAGGTAAGTTAG1022160.24026352830385503No Hit
TCACTCTCAAATGAAACAATTCGATACCAAGTACTCTGGTAATAGAATTT1017520.2391728744225352No Hit
GCTCCATTTAGGCAGGACAGTATTTTGATAATATACCCCAGATCTCAAAC993190.2334539931870801No Hit
GGACACGATGAAGTTGGATATAAAGAAAACTTTTAGTAATAGATCCGATC988550.23236333930576025No Hit
CTTGATGCTGCCTGTATTTTTTGCAAGATTATTAAAAGCGAAATTCCATC873180.2052450767437193No Hit
AGGGACATTAAGCAGGATGCTGTAAGAGAATTTTTTGCATCTCAAGTAGG866010.20355973443142122No Hit
CCGCTGGTGCTAATGTCGACAACGTATGTACAAAAATGCAACCTAAAACG848380.19941571979183742No Hit
GTTGCTACTGATGTGGCCGCCAGAGGTATCGGTATGTTAAAATTTTCTCC777160.1826751229324411No Hit
TTTCTCTGGCTTCCCAGTTACTGGTATGTTATAATAGACAAAATTTTTGG758930.1783900754633763No Hit
CAATTAATCAATATACGCAGAGATGGTCAGTATAACATGATTTTATAACT738530.1735949592610218No Hit
GCTGACAAGTCATACATTGAAGGGTATGTTCCGATTTAGTTTACTTTATA730490.17170511934597624No Hit
GTTGGCTGCTAACACTCACTTAGGTGCTAGAAACGTTCAAGTATGTTATA729470.1714653635358585No Hit
CTAGAGTTAGAAGCCCCAATTAAAATATGTGGTGACATTCATGGGCAATA707690.1663458718256977No Hit
AATGGACCCACATAATCCAATTGTCCTTGATCAGGGTACTGGTTTCGTCA677530.15925662159711876No Hit
TGTGTCTACAGCGTCTTCAGGAAGAAAGAAAAAAATGGAGAAAGGATCAT649280.15261632587572102No Hit
TGGTACCTACCTGGGTTGCAGCAGCTTGGGTCAAACATTTGTCAAAGGAA634120.1490528963841674No Hit
TGTCAGACCCTGTAGAGTTATTGAAAAGAGCTGAGAAGAAGGGTGTTCCT629350.14793168538979334No Hit
CGGTGCTCAAGGTACTAAGTTTAGAATCTCAGTATGTTAAAATTTTTATT592360.13923701145228887No Hit
CCACTGAATTAACCGTTCAATCCGAGAGAGCATTCCAAAAGCAACCACAC566070.13305742297386244No Hit
AAAACACACCAGATAATTAGTGCATATATATTAGATCAATATGGTATGTG542500.12751718332241663No Hit
CATGTCAGACTACGAGGAGGCGTATGTGCATTCATATTGGAGAAATTGCG536430.12609040119750034No Hit
GTCAAGATCTCGAGACTAGCAATAACAAAATGGTATGTTAATATGGACTA518810.12194873710507459No Hit
TGAAAAAGGGCGAAGTCAGTTTAGGTACCTCAATGTATGTATATAAGAAT506700.11910222449671613No Hit
AGAGCATCCAGACCAAAACGTATCTCTAACAGAAAAATCAGCCTCATTAA495350.11643435347236694No Hit
AATGGGTCGTATGCACAGTGCCGGTAAAGGTATTTCTTCTTCTGCTATTC456280.10725076572599493No Hit
TCACTCTCAAATGAAACAATTCGATACCGTATGTAAGATGGTTTTATTGG452400.10633875342868437No Hit
CAATGAACCTCAAATCAATTTTTTGACAGAATATGTTAGAAAAGGCGCTT447160.10510706671788353No Hit
AATACATTGGACAGAAATTATGGACGATGGACGGAAAAAAGAATTGCATG437330.10279647886155291No Hit
AGTAATAAGCTCTGATCGTTTTGAAAAATGAACTTCAATACACCTCAACA435260.10230991559984343No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)