FastQCFastQC Report
Wed 30 Jan 2019
1A_S50_R2_filt.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename1A_S50_R2_filt.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6950949
Sequences flagged as poor quality0
Sequence length23-98
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTATTCTAAGTGCCTCCGCTCTAGCTGGTATGTACACATTCTCCTTTTT3625355.2156187593953No Hit
AAATGGGTCGTATGCACAGTGCCGGTAAAGGTATTTCTTCTTCTGCTATT1985572.8565451997993367No Hit
CAAGTCTTTTAAGGAAAAACAGTGCGGAAAAATGGCTCACTTTAAAGAAT1163031.6731959909359138No Hit
GCTGACAAGTCATACATTGAAGGTACTGCTGTTTCTCAAGCTGACGTCAC1051651.5129588779891783No Hit
CCACTGAATTAACCGTTCAATCCGAGAGAGCTTTCCAAAAGCAACCACAC1046691.5058231617006541No Hit
GAAAGCAACAAGACAACTAAGACTAAGCAACAATGCCAAGAGCTCCAAGA961861.3837822720322075No Hit
CCGCTGGTGCTAATGTCGACAACGTCTGGGCTGATGTTTACGCTAAGGCT886341.2751352369295186No Hit
GTTGGCTGCTAACACTCACTTAGGTGCTAGAAACGTTCAAGTTCACCAAG882521.269639584465373No Hit
TCACTCTCAAATGAAACAATTCGATACCAAGTACTCTGGTAATAGAATTT857971.234320666142134No Hit
GTCAAGATCTCGAGACTAGCAATAACAAAATGGGTAGAGTTAGAACCAAG713431.0263778370406689No Hit
CAATTAATCAATATACGCAGAGATGGCCCCAGTTAAATCCCAAGAATCTA603470.8681836106120185No Hit
AAAACACACCAGATAATTAGTGCATATATATTAGATCAATATGGTTTTAG480710.6915746324710482No Hit
AAGAAACCATTAGATCAATAAGCAATGGGTAGAGTTATTCGTAACCAAAG450570.6482136467984443No Hit
CGGTGCTCAAGGTACTAAGTTTAGAATCTCATTAGGTCTACCAGTCGGTG289090.41590004472770553No Hit
AAATGGGTCGTATGCACAGTGCCGTATGTTTATTAACACCATAGCGAGAT278390.4005064632181879No Hit
GTTGCTACTGATGTGGCCGCCAGAGGTATCGATGTCAAAGGTATCAATTA271890.3911552221142753No Hit
CGAACCCATTTCTTTGTCCACCACTTTCAAACAATCTTCTCCAGCTAACC268000.385558863976703No Hit
GCTATTCTAAGTGCCTCCGCTCTAGCTGCTAACTCAACTACTTCTATTCC226720.32617128970447057No Hit
CAATAACAATTAAGAATGGCTAACGGTACGTGAAAGGGGTGATATCCTGT199840.28750031110859825No Hit
ATTTACTGAATTAACAATGGATTCTGAGGTTGCTGCTTTGGTTATTGATA197930.2847524848765255No Hit
CGTATTGCTAAAGAACTAAGTGATCTAGAAAGAGATCCACCTACTTCATG197270.283802974241359No Hit
CGATAAAGAACCAAATAGGACTAAAAATGGCCGGCGTTAAAGCTTACGAA175240.252109460161483No Hit
CAGCATCTCATAATATGTCTGCAACTGCTAAACACGACAGTAACGCTAGC136630.1965630880042423No Hit
AGGGACATTAAGCAGGATGCTGTAAGAGTATGTTAATACGTGAAATGAGA134960.19416053836677552No Hit
CCGTAGAATAGGTACAGTGAGACAGTATATTCGAAATGGCCGGTGTTAAA132370.19043442844998576No Hit
CAATAACAATTAAGAATGGCTAACGACCTTGTTCAAGCTCGCGATAACTC128580.18498193556016596No Hit
CCACTGAATTAACCGTTCAATCCGAGAGAGCTTTCCAAAAGGTATGAATT120370.17317059871968563No Hit
AGCTGTTCAGGTTAGATGCAGGCTACCAACAATACGACTGGGGTAAAATC118090.16989047107092858No Hit
AAAACACACCAGATAATTAGTGCATATATATTAGATCAATATGGTATGTG109330.1572878753678095No Hit
TCCATGCCAGAAGGAGGCCTGCGCCATCCAAGGTTTGTAATATTAACTTC96330.13858539315998433No Hit
GTTGGCTGCTAACACTCACTTAGGTGCTAGAAACGTTCAAGTATGTTATA87830.12635684710102174No Hit
GTTGCTACTGATGTGGCCGCCAGAGGTATCGGTATGTTAAAATTTTCTCC85030.12232862016395173No Hit
AGGGACATTAAGCAGGATGCTGTAAGAGAATTTTTTGCATCTCAAGTAGG83440.12004116272468694No Hit
CAAGTCTTTTAAGGAAAAACAGTGCGGAAAAAGTGAGTAAATACCTACTA80440.11572520529211192No Hit
GTTGGGATATTTTTGGTTGGTGTATGTTATCACCCTGTTCACGTTTTTCG72950.10494969823544957No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)