FastQCFastQC Report
Wed 16 Jan 2019
trim001D_S53_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenametrim001D_S53_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77255243
Sequences flagged as poor quality0
Sequence length67
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGACAGCCTTCTTAATCAAGTAGTACAAATCTTCTGGGATTTCTGGAGCCAAACCATTGGACTTC9667781.2514076229104605No Hit
TTGCACTAGGTTGCTATGATCCTCTCTGTTCTGCAGTTGTCAGTCTTTATTATTTTGTTCATCTCAA7368920.9538407639207089No Hit
AAAAAGCCTTTTATAATTTTTGCATAGCAGCAATATCGGTAGATTGGACGTGGTCTTCGTCTTCTTC6402410.828734691831854No Hit
CTGAAAGCTACTCCCTCTTGAAAGGATACAAAATTAATTGATCCTTCCACAACTTCAGTATTGGATG5241150.6784199746805535No Hit
ACATTATCGCTAAATTCATACCTTTTGGAAAGCTCTCTCGGATTGAACGGTTAATTCAGTGGAGATC3947890.511019038539559No Hit
CGTCTTTGTCCTTTCTGTTTCTTTCCAAGTGCTTTCTAACAGAGACAGCCTTCTTAATCAAGTAGTA2730560.35344656154922716No Hit
CAAGCTGGAGCATTTCTAGAGTATGGAATAGCAGAAGAAGAAATACCTTTACCGGCACTGTGCATAC2637010.3413373510455465No Hit
ATTTCTGGAGCCAAACCATTGGACTTCAAGATTCTCATGATCTTGTTACCAGTGATAACACGAGCTT2380520.30813701537383037No Hit
CTCGCTATGGTGTTAATAAACATACGGCACTGTGCATACGACCCATTTAGATCGGAAGAGCACACGT2364280.3060348926738862No Hit
GTATCTGGCCAATCTGTGAATTCTAGATTCGATCAAAATCAATCTGAACTTAGCGTCTTTGTCCTTT2316910.2999032699955393No Hit
TGACCAAAGCGGAGGCAGTGGCGGATTCGTACTTCCAGTTTGGTGGTAAGACAGCAACAGTTCTGTA2273220.2942479903920566No Hit
CTAAATTCATACCTTTTGGAAAGCTCTCTCGGATTGAACGGTTAATTCAGTGGAGATCGGAAGAGCA2234260.2892049669690379No Hit
TACAAAGAATATTTCTCGTATCCACACGAGACGATGCGAGTAACTAAACAAATAGCTCTCATTTCAC2210400.28611650344559786No Hit
GTCTTGGTTCTAACTCTACCCATTTTGTTATTGCTAGTCTCGAGATCTTGACAGATCGGAAGAGCAC2138980.27687182344374994No Hit
AAATTGTTTGTTAGCCTTACCAGCAGCAGCAGAGACCTTGACAACGTTGAATCTAACAGTCTTGGAG2022150.2617492252273415No Hit
TTTCAACAAAAGGAATAAAAAGACTGTAAAAGGGTGTGATGACATTGCGCCATTACGACAATCACAT1937640.250810161842349No Hit
AAATTCTATTAGTCGTAACAATAAACATGCTAAGAATTCAATAGGATACCTGCTAAGTTTTCATCTA1916970.24813461527782651No Hit
TGCAATTTGAAAGAGATACCTCTAACTGGACCCTTTTGGATTCTCTTCATCAAATGGGTGGTGTAAC1901700.24615805039924604No Hit
CCAAATCAGCTTGAGCAGTAGCAGTGGCAGAAGTACCAATACTACATGAAGATGGAATAGAAGTAGT1900040.24594317825134535No Hit
CAACACCACGGGTCTTTGGCCAAGAGTTTCTCTTCAATCTGTACTTGTGGAAAGCACGACCAGCCTT1880650.2434333162346017No Hit
AATTCAAATATGCACAGTTCAGTAGAAAGTGGAAGGTCTCTTGTAGGAGAAAGTCTTGGTGGATTTT1852450.23978307854134895No Hit
GGTTGATGGAAACAATTTCACCAGAAGCCTTCTTAACCTTGTGCAACTTTTGCAAGAAATACCAGTA1800860.2331052146195437No Hit
AGTATCAGAAAAATATTTTATATCTTAAAATTCAAATATGCACAGTTCAGTAGAAAGTGGAAGGTCT1695980.21952943698591432No Hit
GAGTCACCAGCTTCCAAAATAGAGACAACACCGACTCTGAATAACTTACCGACAGCAGTACCCAATT1679920.21745061367550161No Hit
ATGCAACTTTTGCATTAATATCTCGCTATGGTGTTAATAAACATACGGCACTGTGCATACGACCCAT1656760.2144527588891281No Hit
CGATACCTCTGGCGGCCACATCAGTAGCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTG1621920.20994303260427255TruSeq Adapter, Index 2 (97% over 35bp)
ACGTAAGTCTTATGCTGATTCATAACCAGCTTAAAATTTAGCAAATTGTTTGTTAGCCTTACCAGCA1614310.20895798619130612No Hit
TTGTATTCCAAGCCTTCTTGCTAATCCCATGAAAGAATAAAGCGAAATAGTTATAAAATCATGTTAT1474640.19087895432546886No Hit
GAAGAGGACCACGAATCTACTCGAGATTTGTGAGGAAAGAGGCAAATAAAGAAAAAAAGATGAAAAA1423790.18429687678284826No Hit
GAGATGTTTAAAAAATCAATTTTAGTTATACAAAATATACATATAAAATAATGAAATAAATGATTTA1401840.18145564567054692No Hit
GACAACTTGAACCAAGCTGGAGCATTTCTAGAGTATGGAATAGCAGAAGAAGAAATACCTTTACCGG1335240.17283487154392874No Hit
ATGTATGACTTGTCAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG1328940.17201939291032972TruSeq Adapter, Index 4 (100% over 49bp)
AACCGAATTCACCGGCCAACTTCAATTCTGCGTCCAAACGAGAAGATTCGTAAGGTCTCTTTGGTGT1246680.1613715718944797No Hit
GAAAGCTCTCTCGGATTGAACGGTTAATTCAGTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTC1228560.15902610001498538Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGGCGATAATGTACAAGTTTCTGGCACCACTGTTGTCAGCACAGTTCATGATGGCACCGACTGGTAG1224290.1584733867188794No Hit
GCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAA1185710.15347955089598256TruSeq Adapter, Index 4 (100% over 53bp)
ACGAATTCTGATGGAGAGTCAAGATCAGTTAGAGCAATGACAGGGATGTTAACGTAAGAAGCTTCCT1095940.14185962757246134No Hit
ATGAAGAGGACCACGAATCTACTCGAGATTTGTGAGGAAAGAGGCAAATAAAGAAAAAAAGATGAAA1056060.1366975183807266No Hit
TCAACTTTGGATAGTAACGTTCAATCAAAGCCTTAGAAGCACGCTTGACGGTCTTGGTTCTAACTCT1055170.13658231584359912No Hit
GAAATAGCTGTAATATCGTTAGGGGTAACAAAAAAAAATAAAATCAATAAAAAAATGGAGAAAATTT1043090.13501866792393624No Hit
TCTTAGATCTTTTTCACACCTTGGAAAACTTTATGGATGATTAAAAAGCGCATTCTAATGAAAGAAA1041190.134772729923327No Hit
TTCTCATGATCTTGTTACCAGTGATAACACGAGCTTGGGTAACACCGTGAGCATCTCTCAACAAGAC1003020.12983196493214058No Hit
CTAGCCAATAAGTACCAGATTAAACCAATGGAGTGCTTACCTCTGTTGTTACATGGGATAGCGACAT993270.1285699146658564No Hit
CTCTGGCGGCCACATCAGTAGCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAAT966380.12508924475197108TruSeq Adapter, Index 4 (100% over 41bp)
GGCCAAACCCAACTTGTAGACTTGAGTTTGCAATCTTCTTTCCAAGAAATCTTCAACCTTCAAAGCC956200.12377153483291742No Hit
TGCTTTCTAACAGAGACAGCCTTCTTAATCAAGTAGTACAAATCTTCTGGGATTTCTGGAGCCAAAC952860.12333920171605699No Hit
AGATGTTTAAAAAATCAATTTTAGTTATACAAAATATACATATAAAATAATGAAATAAATGATTTAA936140.1211749473106958No Hit
AGAGATGTTTAAAAAATCAATTTTAGTTATACAAAATATACATATAAAATAATGAAATAAATGATTT930640.12046302151945856No Hit
GGGTCTCTGTAGAAGTACAAATCTGGCATGATGGACCATGGTTGAGTTCTGTCAACTAAAGCACCTC929350.12029604256115019No Hit
CACCGGTGATTTTCTTGATAGAGTCAGCGGAGAAAGAAGATAAAGAACTGGAGTTGAAGATAGTCAA923510.1195401068119092No Hit
AGAGTGACAATGGACTTAGCAGCTGGCTTAGCTGGCTTAGCAGCCTTCTTAGCGTTGTATGCGGCAA922120.1193601837483056No Hit
AATTCTAGATTCGATCAAAATCAATCTGAACTTAGCGTCTTTGTCCTTTCTGTTTCTTTCCAAGTGC907730.11749752699632308No Hit
GCCAAAACCAATTTTTCCCAGGTCTTACCAACGTTGATAACGTGGACACCATCTGGTCTTGCGTTGA902630.11683737762626673No Hit
CTTACAAAGAATATTTCTCGTATCCACACGAGACGATGCGAGTAACTAAACAAATAGCTCTCATTTC882290.11420454660921847No Hit
CCTTCTTAACCTTGTGCAACTTTTGCAAGAAATACCAGTAACGAGACTTGGCAATAACTTCATTTGA876390.11344084439680036No Hit
TGAACCAAGCTGGAGCATTTCTAGAGTATGGAATAGCAGAAGAAGAAATACCTTTACCGGCACTGTG862220.11160666467646732No Hit
GCAGCGGCAGCAGAGGCAGCTGGGAAAGAGTCGAATTCATCGGCCTTGGAAGCGATGTGGTTGAACC835610.10816223825740863No Hit
CTTGTTTTCGTCTGGGTTGTGACCGATGATAATAACGTAGTTACCGGAAGCTCTGGCTAGGGCACCT801870.10379489713080055No Hit
GTTGTTGATGTTAAAAACATTAACCTTCTTCAAAGTGGAGAAACCTTCGACACTTTCCAAAGTGGTG797150.10318393536086605No Hit
ATTATTCTGTTTCATTACTCGCCCTAATCCTATGACAGGCAGAAATATTGAAGAATATTCCAAAACC794290.1028137339494227No Hit
ACCTTCTTCAAAGTGGAGAAACCTTCGACACTTTCCAAAGTGGTGTCAGAAACAATAATGTTGTTAG784710.10157368866214038No Hit
CGTTGTATGCGGCAATTCTTTCAGCCTTCAACTTTTCAGCTTCAGCGTCAGCTTCTTCATCGTCGGA778760.10080351439707463No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)