FastQCFastQC Report
Wed 16 Jan 2019
trim001C_S52_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenametrim001C_S52_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83068249
Sequences flagged as poor quality0
Sequence length67
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGACAGCCTTCTTAATCAAGTAGTACAAATCTTCTGGGATTTCTGGAGCCAAACCATTGGACTTC14174471.7063643655231016No Hit
AAAAAGCCTTTTATAATTTTTGCATAGCAGCAATATCGGTAGATTGGACGTGGTCTTCGTCTTCTTC9605791.1563732371438333No Hit
CTGAAAGCTACTCCCTCTTGAAAGGATACAAAATTAATTGATCCTTCCACAACTTCAGTATTGGATG6301530.7585967052224731No Hit
CGTCTTTGTCCTTTCTGTTTCTTTCCAAGTGCTTTCTAACAGAGACAGCCTTCTTAATCAAGTAGTA4129780.4971550561996317No Hit
GTATCTGGCCAATCTGTGAATTCTAGATTCGATCAAAATCAATCTGAACTTAGCGTCTTTGTCCTTT3797630.4571698628196677No Hit
TGACCAAAGCGGAGGCAGTGGCGGATTCGTACTTCCAGTTTGGTGGTAAGACAGCAACAGTTCTGTA3787750.455980479376663No Hit
ATTTCTGGAGCCAAACCATTGGACTTCAAGATTCTCATGATCTTGTTACCAGTGATAACACGAGCTT3257070.39209566100279786No Hit
AAATTGTTTGTTAGCCTTACCAGCAGCAGCAGAGACCTTGACAACGTTGAATCTAACAGTCTTGGAG3164010.3808928246459125No Hit
AATTCAAATATGCACAGTTCAGTAGAAAGTGGAAGGTCTCTTGTAGGAGAAAGTCTTGGTGGATTTT3085750.37147165579474295No Hit
CCAGGTAGGTACCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCG2861130.3444312399073201TruSeq Adapter, Index 3 (100% over 52bp)
CCAAATCAGCTTGAGCAGTAGCAGTGGCAGAAGTACCAATACTACATGAAGATGGAATAGAAGTAGT2810630.33835190145876315No Hit
ACGTAAGTCTTATGCTGATTCATAACCAGCTTAAAATTTAGCAAATTGTTTGTTAGCCTTACCAGCA2720460.32749697179725074No Hit
AGTATCAGAAAAATATTTTATATCTTAAAATTCAAATATGCACAGTTCAGTAGAAAGTGGAAGGTCT2647550.3187198516728094No Hit
ATGTATGACTTGTCAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG2456410.2957098566023704TruSeq Adapter, Index 3 (100% over 49bp)
GTTGTTGATGTTAAAAACATTAACCTTCTTCAAAGTGGAGAAACCTTCGACACTTTCCAAAGTGGTG2300720.2769674367398788No Hit
CTCTGGCGGCCACATCAGTAGCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCAT2299320.2767989006244732TruSeq Adapter, Index 3 (100% over 41bp)
GAGTCACCAGCTTCCAAAATAGAGACAACACCGACTCTGAATAACTTACCGACAGCAGTACCCAATT2279620.2744273567148382No Hit
GGTTGATGGAAACAATTTCACCAGAAGCCTTCTTAACCTTGTGCAACTTTTGCAAGAAATACCAGTA2265380.27271310365528467No Hit
GAGCGGAGGCACTTAGAATAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTC2017350.24285452315240208TruSeq Adapter, Index 3 (100% over 44bp)
ACCTTCTTCAAAGTGGAGAAACCTTCGACACTTTCCAAAGTGGTGTCAGAAACAATAATGTTGTTAG1984090.23885058658212477No Hit
TAAATATCACCTTACTTACCACTCGACGTTGTCAGCATTTTCTTCAGCCCATTCAGTAGCTTCAGCT1959780.23592407732104717No Hit
TGCAATTTGAAAGAGATACCTCTAACTGGACCCTTTTGGATTCTCTTCATCAAATGGGTGGTGTAAC1886640.22711926912050354No Hit
CAAGCTGGAGCATTTCTAGAGTATGGAATAGCAGAAGAAGAAATACCTTTACCGGCACTGTGCATAC1861450.2240868228725996No Hit
ACGAATTCTGATGGAGAGTCAAGATCAGTTAGAGCAATGACAGGGATGTTAACGTAAGAAGCTTCCT1701800.20486768656938947No Hit
GAGATGTTTAAAAAATCAATTTTAGTTATACAAAATATACATATAAAATAATGAAATAAATGATTTA1665770.20053028925648836No Hit
TACAAAGAATATTTCTCGTATCCACACGAGACGATGCGAGTAACTAAACAAATAGCTCTCATTTCAC1654350.19915551608653748No Hit
CAACACCACGGGTCTTTGGCCAAGAGTTTCTCTTCAATCTGTACTTGTGGAAAGCACGACCAGCCTT1647450.19832487380346733No Hit
GTCTTGGTTCTAACTCTACCCATTTTGTTATTGCTAGTCTCGAGATCTTGACAGATCGGAAGAGCAC1534890.184774570124862No Hit
GGCCAAACCCAACTTGTAGACTTGAGTTTGCAATCTTCTTTCCAAGAAATCTTCAACCTTCAAAGCC1517580.1826907414408121No Hit
CTCGCTATGGTGTTAATAAACATACGGCACTGTGCATACGACCCATTTAGATCGGAAGAGCACACGT1505030.18117993554914105No Hit
GGGTCTCTGTAGAAGTACAAATCTGGCATGATGGACCATGGTTGAGTTCTGTCAACTAAAGCACCTC1470420.17701348201043698No Hit
AGAGTGACAATGGACTTAGCAGCTGGCTTAGCTGGCTTAGCAGCCTTCTTAGCGTTGTATGCGGCAA1449130.17445052922687707No Hit
AATTCTAGATTCGATCAAAATCAATCTGAACTTAGCGTCTTTGTCCTTTCTGTTTCTTTCCAAGTGC1422200.17120861666411194No Hit
CTAGCCAATAAGTACCAGATTAAACCAATGGAGTGCTTACCTCTGTTGTTACATGGGATAGCGACAT1413920.17021184592442776No Hit
CACCGGTGATTTTCTTGATAGAGTCAGCGGAGAAAGAAGATAAAGAACTGGAGTTGAAGATAGTCAA1292570.15560337620695483No Hit
CGTTGTATGCGGCAATTCTTTCAGCCTTCAACTTTTCAGCTTCAGCGTCAGCTTCTTCATCGTCGGA1254260.15099150579182186No Hit
TTGGTCATGTATGGAGTAGTTGCTCTTTGGTCTTTTGGGATGGCCTTTTCCTTAAGTGTCTTTCTTC1239200.14917853872181655No Hit
CGATACCTCTGGCGGCCACATCAGTAGCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTT1232200.14833585814478886TruSeq Adapter, Index 1 (97% over 35bp)
TGCTTTCTAACAGAGACAGCCTTCTTAATCAAGTAGTACAAATCTTCTGGGATTTCTGGAGCCAAAC1231990.14831057772747802No Hit
CTTCCAAATTGGTTTCATCATCCCATGGCTTGACATCTAGAGTGACAATGGACTTAGCAGCTGGCTT1183200.14243709410559502No Hit
TTGCACTAGGTTGCTATGATCCTCTCTGTTCTGCAGTTGTCAGTCTTTATTATTTTGTTCATCTCAA1176190.14159320969917158No Hit
TTCTCATGATCTTGTTACCAGTGATAACACGAGCTTGGGTAACACCGTGAGCATCTCTCAACAAGAC1173680.14129104852083735No Hit
AGAGATGTTTAAAAAATCAATTTTAGTTATACAAAATATACATATAAAATAATGAAATAAATGATTT1168210.14063255384136No Hit
AACCGAATTCACCGGCCAACTTCAATTCTGCGTCCAAACGAGAAGATTCGTAAGGTCTCTTTGGTGT1142860.137580846323124No Hit
AGATGTTTAAAAAATCAATTTTAGTTATACAAAATATACATATAAAATAATGAAATAAATGATTTAA1112870.13397056196525822No Hit
TCTGGCAGCCATGTCTTGGTATAAGGTTTCGACGGCAGCAACTCTGGAGACGTCTCTGATTTCCTTG1111350.13378757989710366No Hit
GAAATAGCTGTAATATCGTTAGGGGTAACAAAAAAAAATAAAATCAATAAAAAAATGGAGAAAATTT1097770.1321527795776699No Hit
TCAAATCACCGGTGATTTTCTTGATAGAGTCAGCGGAGAAAGAAGATAAAGAACTGGAGTTGAAGAT1075510.12947305534272185No Hit
ATTGAATTTATATTTTTAGTACATTATACAAATAATTATAAAATAAACAGTTACGTAAGTCTTATGC1030050.12400044690962489No Hit
CCTTCTTAACCTTGTGCAACTTTTGCAAGAAATACCAGTAACGAGACTTGGCAATAACTTCATTTGA1029100.12388608311702826No Hit
GCAGCGGCAGCAGAGGCAGCTGGGAAAGAGTCGAATTCATCGGCCTTGGAAGCGATGTGGTTGAACC1020820.12288931237734407No Hit
ATCTTAAAATTCAAATATGCACAGTTCAGTAGAAAGTGGAAGGTCTCTTGTAGGAGAAAGTCTTGGT1012800.1219238412019495No Hit
ATGCAACTTTTGCATTAATATCTCGCTATGGTGTTAATAAACATACGGCACTGTGCATACGACCCAT1011160.12172641318104586No Hit
GACAACTTGAACCAAGCTGGAGCATTTCTAGAGTATGGAATAGCAGAAGAAGAAATACCTTTACCGG1008860.12144953242002247No Hit
CTTGAGCATCTGATCTTGGGTCGGTGACAATAACTAATCTTGGTTCCTTGAAAGAACGGGTGATATA962850.11591071337015903No Hit
CGGCGATAATGTACAAGTTTCTGGCACCACTGTTGTCAGCACAGTTCATGATGGCACCGACTGGTAG949410.11429276666226587No Hit
TTATTGAATTTATATTTTTAGTACATTATACAAATAATTATAAAATAAACAGTTACGTAAGTCTTAT939740.1131286636365719No Hit
TCAATCTGAACTTAGCGTCTTTGTCCTTTCTGTTTCTTTCCAAGTGCTTTCTAACAGAGACAGCCTT939040.11304439557886914No Hit
AGAACTGACGTAGCAACTGAATTAACGAACCTTTACCGAAAGAGTTGGTAATAGATGCGTGAAACGC938770.11301189218518377No Hit
ATGTTCAAATCACCGGTGATTTTCTTGATAGAGTCAGCGGAGAAAGAAGATAAAGAACTGGAGTTGA934290.11247257661588606No Hit
GAAAGCTCTCTCGGATTGAACGGTTAATTCAGTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTC878740.10578530432247345Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GAGTTCTGTCAACTAAAGCACCTCTTAGTCTCAAAACTTCTCTAGCCAATAAGTACCAGATTAAACC871630.10492938162209246No Hit
TATTGAATTTATATTTTTAGTACATTATACAAATAATTATAAAATAAACAGTTACGTAAGTCTTATG869900.10472111913662704No Hit
TTGAATTTATATTTTTAGTACATTATACAAATAATTATAAAATAAACAGTTACGTAAGTCTTATGCT866050.10425764481926181No Hit
TCAACTTTGGATAGTAACGTTCAATCAAAGCCTTAGAAGCACGCTTGACGGTCTTGGTTCTAACTCT854290.1028419414498553No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)