FastQCFastQC Report
Wed 16 Jan 2019
trim001B_S51_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenametrim001B_S51_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106240399
Sequences flagged as poor quality0
Sequence length67
%GC36

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGACAGCCTTCTTAATCAAGTAGTACAAATCTTCTGGGATTTCTGGAGCCAAACCATTGGACTTC17730541.6689075122920047No Hit
AAAAAGCCTTTTATAATTTTTGCATAGCAGCAATATCGGTAGATTGGACGTGGTCTTCGTCTTCTTC9800400.922473945151505No Hit
CTGAAAGCTACTCCCTCTTGAAAGGATACAAAATTAATTGATCCTTCCACAACTTCAGTATTGGATG7808350.7349699430251575No Hit
CGTCTTTGTCCTTTCTGTTTCTTTCCAAGTGCTTTCTAACAGAGACAGCCTTCTTAATCAAGTAGTA6617960.6229231123275432No Hit
AATTCAAATATGCACAGTTCAGTAGAAAGTGGAAGGTCTCTTGTAGGAGAAAGTCTTGGTGGATTTT5502850.517962098391592No Hit
GTATCTGGCCAATCTGTGAATTCTAGATTCGATCAAAATCAATCTGAACTTAGCGTCTTTGTCCTTT4991740.46985328057738185No Hit
AGTATCAGAAAAATATTTTATATCTTAAAATTCAAATATGCACAGTTCAGTAGAAAGTGGAAGGTCT4631310.43592739142480064No Hit
GGTTGATGGAAACAATTTCACCAGAAGCCTTCTTAACCTTGTGCAACTTTTGCAAGAAATACCAGTA4567420.4299136715403338No Hit
GAGTCACCAGCTTCCAAAATAGAGACAACACCGACTCTGAATAACTTACCGACAGCAGTACCCAATT4135220.3892323484214324No Hit
ATTTCTGGAGCCAAACCATTGGACTTCAAGATTCTCATGATCTTGTTACCAGTGATAACACGAGCTT3947080.3715234540864253No Hit
AAATTGTTTGTTAGCCTTACCAGCAGCAGCAGAGACCTTGACAACGTTGAATCTAACAGTCTTGGAG3912520.3682704542553535No Hit
TGCAATTTGAAAGAGATACCTCTAACTGGACCCTTTTGGATTCTCTTCATCAAATGGGTGGTGTAAC3615680.34033004714148335No Hit
TGACCAAAGCGGAGGCAGTGGCGGATTCGTACTTCCAGTTTGGTGGTAAGACAGCAACAGTTCTGTA3518440.33117722007049316No Hit
ACGTAAGTCTTATGCTGATTCATAACCAGCTTAAAATTTAGCAAATTGTTTGTTAGCCTTACCAGCA3340490.31442747122965903No Hit
GAGATGTTTAAAAAATCAATTTTAGTTATACAAAATATACATATAAAATAATGAAATAAATGATTTA3112350.2929535307938744No Hit
GGCCAAACCCAACTTGTAGACTTGAGTTTGCAATCTTCTTTCCAAGAAATCTTCAACCTTCAAAGCC2478970.23333590831111245No Hit
CAACACCACGGGTCTTTGGCCAAGAGTTTCTCTTCAATCTGTACTTGTGGAAAGCACGACCAGCCTT2332670.2195652521975186No Hit
CCTTCTTAACCTTGTGCAACTTTTGCAAGAAATACCAGTAACGAGACTTGGCAATAACTTCATTTGA2290790.2156232489300045No Hit
ATTGAATTTATATTTTTAGTACATTATACAAATAATTATAAAATAAACAGTTACGTAAGTCTTATGC2203080.20736744409252453No Hit
TTGAATTTATATTTTTAGTACATTATACAAATAATTATAAAATAAACAGTTACGTAAGTCTTATGCT2067540.19460958538003986No Hit
ACGAATTCTGATGGAGAGTCAAGATCAGTTAGAGCAATGACAGGGATGTTAACGTAAGAAGCTTCCT2042510.1922536077824783No Hit
AGATGTTTAAAAAATCAATTTTAGTTATACAAAATATACATATAAAATAATGAAATAAATGATTTAA2036640.1917010872671892No Hit
AGAGATGTTTAAAAAATCAATTTTAGTTATACAAAATATACATATAAAATAATGAAATAAATGATTT2035140.19155989803841003No Hit
AACCGAATTCACCGGCCAACTTCAATTCTGCGTCCAAACGAGAAGATTCGTAAGGTCTCTTTGGTGT2002640.18850079808152828No Hit
TCTGGCAGCCATGTCTTGGTATAAGGTTTCGACGGCAGCAACTCTGGAGACGTCTCTGATTTCCTTG1915560.18030429272013557No Hit
TGCTTTCTAACAGAGACAGCCTTCTTAATCAAGTAGTACAAATCTTCTGGGATTTCTGGAGCCAAAC1900590.17889522421691958No Hit
GTTGTTGATGTTAAAAACATTAACCTTCTTCAAAGTGGAGAAACCTTCGACACTTTCCAAAGTGGTG1887350.17764899395756223No Hit
AGAGTGACAATGGACTTAGCAGCTGGCTTAGCTGGCTTAGCAGCCTTCTTAGCGTTGTATGCGGCAA1849180.17405619871589526No Hit
TATTGAATTTATATTTTTAGTACATTATACAAATAATTATAAAATAAACAGTTACGTAAGTCTTATG1824940.17177458077882407No Hit
AATTCTAGATTCGATCAAAATCAATCTGAACTTAGCGTCTTTGTCCTTTCTGTTTCTTTCCAAGTGC1762720.1659180515690646No Hit
CCAAATCAGCTTGAGCAGTAGCAGTGGCAGAAGTACCAATACTACATGAAGATGGAATAGAAGTAGT1739000.16368537923130352No Hit
ATCTTAAAATTCAAATATGCACAGTTCAGTAGAAAGTGGAAGGTCTCTTGTAGGAGAAAGTCTTGGT1724110.1622838408202891No Hit
TTATTGAATTTATATTTTTAGTACATTATACAAATAATTATAAAATAAACAGTTACGTAAGTCTTAT1716330.16155153935368785No Hit
CAAGCTGGAGCATTTCTAGAGTATGGAATAGCAGAAGAAGAAATACCTTTACCGGCACTGTGCATAC1708310.16079664761048196No Hit
ACCTTCTTCAAAGTGGAGAAACCTTCGACACTTTCCAAAGTGGTGTCAGAAACAATAATGTTGTTAG1651380.15543804574755032No Hit
TTGGTCATGTATGGAGTAGTTGCTCTTTGGTCTTTTGGGATGGCCTTTTCCTTAAGTGTCTTTCTTC1647610.15508319015255204No Hit
TTCTCATGATCTTGTTACCAGTGATAACACGAGCTTGGGTAACACCGTGAGCATCTCTCAACAAGAC1573280.1480867932357822No Hit
CGTTGTATGCGGCAATTCTTTCAGCCTTCAACTTTTCAGCTTCAGCGTCAGCTTCTTCATCGTCGGA1559620.14680102999236666No Hit
GCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAA1556300.1464885311660021TruSeq Adapter, Index 2 (100% over 53bp)
CTAGCCAATAAGTACCAGATTAAACCAATGGAGTGCTTACCTCTGTTGTTACATGGGATAGCGACAT1543160.1452517135218967No Hit
TACAAAGAATATTTCTCGTATCCACACGAGACGATGCGAGTAACTAAACAAATAGCTCTCATTTCAC1526730.14370522083600232No Hit
CTTGTTTTCGTCTGGGTTGTGACCGATGATAATAACGTAGTTACCGGAAGCTCTGGCTAGGGCACCT1464600.1378571629799696No Hit
AAGAGATGTTTAAAAAATCAATTTTAGTTATACAAAATATACATATAAAATAATGAAATAAATGATT1464560.13785339793386883No Hit
TGAAATGTTGAAATTATTAAATTCTACAGAATTGATAATACATAAAAATAAACTATTAAAGTATCAG1416720.1333504027973389No Hit
GTCTTGGTTCTAACTCTACCCATTTTGTTATTGCTAGTCTCGAGATCTTGACAGATCGGAAGAGCAC1295200.12191219274317673No Hit
GCCAAAACCAATTTTTCCCAGGTCTTACCAACGTTGATAACGTGGACACCATCTGGTCTTGCGTTGA1283190.12078173765141828No Hit
GACAACTTGAACCAAGCTGGAGCATTTCTAGAGTATGGAATAGCAGAAGAAGAAATACCTTTACCGG1261080.11870060841921348No Hit
GGGTCTCTGTAGAAGTACAAATCTGGCATGATGGACCATGGTTGAGTTCTGTCAACTAAAGCACCTC1251270.1177772308629978No Hit
GATTATTGAATTTATATTTTTAGTACATTATACAAATAATTATAAAATAAACAGTTACGTAAGTCTT1226170.1154146644347599No Hit
CTTCCAAATTGGTTTCATCATCCCATGGCTTGACATCTAGAGTGACAATGGACTTAGCAGCTGGCTT1223420.11515581751533144No Hit
TCAACTTTGGATAGTAACGTTCAATCAAAGCCTTAGAAGCACGCTTGACGGTCTTGGTTCTAACTCT1211780.11406018910000518No Hit
CTTCTCCAAGACTTAGCTTGACGGACAACAATAGCTGGCATAACCTTCTTTCTCAATTCTGGCTTAC1203490.11327988329561903No Hit
AAAGAGATGTTTAAAAAATCAATTTTAGTTATACAAAATATACATATAAAATAATGAAATAAATGAT1198790.11283749037877765No Hit
GATGTTTAAAAAATCAATTTTAGTTATACAAAATATACATATAAAATAATGAAATAAATGATTTAAA1196560.11262758905865931No Hit
AATGTTGAAATTATTAAATTCTACAGAATTGATAATACATAAAAATAAACTATTAAAGTATCAGAAA1176750.11076294997724924No Hit
TCAAATCACCGGTGATTTTCTTGATAGAGTCAGCGGAGAAAGAAGATAAAGAACTGGAGTTGAAGAT1163170.10948471682603526No Hit
CACCGGTGATTTTCTTGATAGAGTCAGCGGAGAAAGAAGATAAAGAACTGGAGTTGAAGATAGTCAA1129400.10630607665545382No Hit
ATGTATGACTTGTCAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG1129220.10628913394800032TruSeq Adapter, Index 2 (100% over 49bp)
GAAATGTTGAAATTATTAAATTCTACAGAATTGATAATACATAAAAATAAACTATTAAAGTATCAGA1106420.10414305767055712No Hit
GCAGCGGCAGCAGAGGCAGCTGGGAAAGAGTCGAATTCATCGGCCTTGGAAGCGATGTGGTTGAACC1102270.10375243413760146No Hit
AAAGAGTCGAATTCATCGGCCTTGGAAGCGATGTGGTTGAACCATCTGGAGAATTCTGGGTAAGCAG1071740.100878762701183No Hit
TAAAGAGATGTTTAAAAAATCAATTTTAGTTATACAAAATATACATATAAAATAATGAAATAAATGA1063320.10008622049696933No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)