FastQCFastQC Report
Wed 16 Jan 2019
trim001A_S50_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenametrim001A_S50_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80898093
Sequences flagged as poor quality0
Sequence length67
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGACAGCCTTCTTAATCAAGTAGTACAAATCTTCTGGGATTTCTGGAGCCAAACCATTGGACTTC13843781.711261599207289No Hit
AAAAAGCCTTTTATAATTTTTGCATAGCAGCAATATCGGTAGATTGGACGTGGTCTTCGTCTTCTTC6202420.7666954522648637No Hit
GAGTCACCAGCTTCCAAAATAGAGACAACACCGACTCTGAATAACTTACCGACAGCAGTACCCAATT5204140.6432957572930674No Hit
CGTCTTTGTCCTTTCTGTTTCTTTCCAAGTGCTTTCTAACAGAGACAGCCTTCTTAATCAAGTAGTA4665870.5767589601895807No Hit
GTCTTGGTTCTAACTCTACCCATTTTGTTATTGCTAGTCTCGAGATCTTGACAGATCGGAAGAGCAC4313730.5332301220005273No Hit
CAAGCTGGAGCATTTCTAGAGTATGGAATAGCAGAAGAAGAAATACCTTTACCGGCACTGTGCATAC4257740.5263090688676679No Hit
TGACCAAAGCGGAGGCAGTGGCGGATTCGTACTTCCAGTTTGGTGGTAAGACAGCAACAGTTCTGTA3932990.48616597179861826No Hit
ATTTCTGGAGCCAAACCATTGGACTTCAAGATTCTCATGATCTTGTTACCAGTGATAACACGAGCTT3819390.47212361359370975No Hit
GGTTGATGGAAACAATTTCACCAGAAGCCTTCTTAACCTTGTGCAACTTTTGCAAGAAATACCAGTA3637540.44964471535812345No Hit
GTATCTGGCCAATCTGTGAATTCTAGATTCGATCAAAATCAATCTGAACTTAGCGTCTTTGTCCTTT3584540.4430932630266081No Hit
CTGAAAGCTACTCCCTCTTGAAAGGATACAAAATTAATTGATCCTTCCACAACTTCAGTATTGGATG3243990.4009970914889181No Hit
AATTCAAATATGCACAGTTCAGTAGAAAGTGGAAGGTCTCTTGTAGGAGAAAGTCTTGGTGGATTTT2848930.3521628130344185No Hit
TGCAATTTGAAAGAGATACCTCTAACTGGACCCTTTTGGATTCTCTTCATCAAATGGGTGGTGTAAC2631430.325277136013577No Hit
AAATTGTTTGTTAGCCTTACCAGCAGCAGCAGAGACCTTGACAACGTTGAATCTAACAGTCTTGGAG2485260.3072087249324901No Hit
AGTATCAGAAAAATATTTTATATCTTAAAATTCAAATATGCACAGTTCAGTAGAAAGTGGAAGGTCT2435650.30107631832557535No Hit
ACGTAAGTCTTATGCTGATTCATAACCAGCTTAAAATTTAGCAAATTGTTTGTTAGCCTTACCAGCA1891660.23383245881951753No Hit
CCAAATCAGCTTGAGCAGTAGCAGTGGCAGAAGTACCAATACTACATGAAGATGGAATAGAAGTAGT1758030.2173141460825288No Hit
AACCGAATTCACCGGCCAACTTCAATTCTGCGTCCAAACGAGAAGATTCGTAAGGTCTCTTTGGTGT1736190.2146144532727119No Hit
CCTTCTTAACCTTGTGCAACTTTTGCAAGAAATACCAGTAACGAGACTTGGCAATAACTTCATTTGA1703140.21052906648862538No Hit
GACAACTTGAACCAAGCTGGAGCATTTCTAGAGTATGGAATAGCAGAAGAAGAAATACCTTTACCGG1699020.21001978377908118No Hit
ATGTATGACTTGTCAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG1693410.20932631873040566TruSeq Adapter, Index 1 (100% over 49bp)
GGCCAAACCCAACTTGTAGACTTGAGTTTGCAATCTTCTTTCCAAGAAATCTTCAACCTTCAAAGCC1561990.1930811891944103No Hit
TCAACTTTGGATAGTAACGTTCAATCAAAGCCTTAGAAGCACGCTTGACGGTCTTGGTTCTAACTCT1436280.17754188593790463No Hit
TTCTCATGATCTTGTTACCAGTGATAACACGAGCTTGGGTAACACCGTGAGCATCTCTCAACAAGAC1430710.1768533653815548No Hit
AATTCTAGATTCGATCAAAATCAATCTGAACTTAGCGTCTTTGTCCTTTCTGTTTCTTTCCAAGTGC1348810.16672951734474137No Hit
TGCTTTCTAACAGAGACAGCCTTCTTAATCAAGTAGTACAAATCTTCTGGGATTTCTGGAGCCAAAC1327070.16404218576573862No Hit
TACAAAGAATATTTCTCGTATCCACACGAGACGATGCGAGTAACTAAACAAATAGCTCTCATTTCAC1320700.16325477536287536No Hit
TGAACCAAGCTGGAGCATTTCTAGAGTATGGAATAGCAGAAGAAGAAATACCTTTACCGGCACTGTG1313290.16233880815954463No Hit
TCTGGCAGCCATGTCTTGGTATAAGGTTTCGACGGCAGCAACTCTGGAGACGTCTCTGATTTCCTTG1253020.15488869434783833No Hit
GAGATGTTTAAAAAATCAATTTTAGTTATACAAAATATACATATAAAATAATGAAATAAATGATTTA1244290.15380955889776043No Hit
CGGTCTTGGTTCTAACTCTACCCATTTTGTTATTGCTAGTCTCGAGATCTTGACAGATCGGAAGAGC1222240.15108390750323372No Hit
TAACCAATCTTCTGATCAAGGCATTACCTTCGAACAATCTCTTTGGGTCCTTTTCGTCTCTAGTTAA1205330.1489936233725559No Hit
CGTTGTATGCGGCAATTCTTTCAGCCTTCAACTTTTCAGCTTCAGCGTCAGCTTCTTCATCGTCGGA1200660.1484163538935337No Hit
TTGGTCATGTATGGAGTAGTTGCTCTTTGGTCTTTTGGGATGGCCTTTTCCTTAAGTGTCTTTCTTC1183700.14631988914744876No Hit
CGGCGATAATGTACAAGTTTCTGGCACCACTGTTGTCAGCACAGTTCATGATGGCACCGACTGGTAG1162610.1437129055687382No Hit
GCCAAAACCAATTTTTCCCAGGTCTTACCAACGTTGATAACGTGGACACCATCTGGTCTTGCGTTGA1039980.12855432822130924No Hit
GAGCGGAGGCACTTAGAATAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTC1035420.12799065609618265TruSeq Adapter, Index 1 (100% over 44bp)
AGAGTGACAATGGACTTAGCAGCTGGCTTAGCTGGCTTAGCAGCCTTCTTAGCGTTGTATGCGGCAA1002930.12397449220465555No Hit
TAACGTAAAGTCAAGATACCTCTCTTGGATTGAGCTTCATCACCAACGTAGGAGTCTTTTTGACCCA986420.12193365299723444No Hit
TCTTGTTACCAGTGATAACACGAGCTTGGGTAACACCGTGAGCATCTCTCAACAAGACACCAATTTG978600.12096700474756555No Hit
AGAGATGTTTAAAAAATCAATTTTAGTTATACAAAATATACATATAAAATAATGAAATAAATGATTT961670.11887424837072487No Hit
CTTCCAAATTGGTTTCATCATCCCATGGCTTGACATCTAGAGTGACAATGGACTTAGCAGCTGGCTT933300.115367367188742No Hit
GCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAA931670.11516587912647087TruSeq Adapter, Index 1 (100% over 53bp)
ACGAATTCTGATGGAGAGTCAAGATCAGTTAGAGCAATGACAGGGATGTTAACGTAAGAAGCTTCCT922670.11405336835319467No Hit
GCAGCGGCAGCAGAGGCAGCTGGGAAAGAGTCGAATTCATCGGCCTTGGAAGCGATGTGGTTGAACC913630.1129359130875928No Hit
ATCTTAAAATTCAAATATGCACAGTTCAGTAGAAAGTGGAAGGTCTCTTGTAGGAGAAAGTCTTGGT911180.11263306293264541No Hit
AATCTGTACTTGTATGGGTCACGGAAGACAACCTTGGCCAATGGGGCACCTCTACCGGAGTCGTGGA897760.11097418575738245No Hit
ACGTCAGCTTGAGAAACAGCAGTACCTTCAATGTATGACTTGTCAGCAGATCGGAAGAGCACACGTC862740.1066452827262566No Hit
CAGCACCTTGTCTCAATCTGGTGTGGGAGGTAAAGATAGAACCAGCACCCTTTCTTTGGTTACGAAT862230.10658224044910428No Hit
TCAATCTGAACTTAGCGTCTTTGTCCTTTCTGTTTCTTTCCAAGTGCTTTCTAACAGAGACAGCCTT861920.10654392063358033No Hit
TAAATATCACCTTACTTACCACTCGACGTTGTCAGCATTTTCTTCAGCCCATTCAGTAGCTTCAGCT860310.10634490481747202No Hit
AAAGAGTCGAATTCATCGGCCTTGGAAGCGATGTGGTTGAACCATCTGGAGAATTCTGGGTAAGCAG850520.10513474032076381No Hit
ACCTTCTTCAAAGTGGAGAAACCTTCGACACTTTCCAAAGTGGTGTCAGAAACAATAATGTTGTTAG823810.10183305557029633No Hit
GTATGGAATAGCAGAAGAAGAAATACCTTTACCGGCACTGTGCATACGACCCATTTAGATCGGAAGA819740.10132995347615921No Hit
TATTGAATTTATATTTTTAGTACATTATACAAATAATTATAAAATAAACAGTTACGTAAGTCTTATG817110.10100485310574626No Hit
AAGAGATGTTTAAAAAATCAATTTTAGTTATACAAAATATACATATAAAATAATGAAATAAATGATT812180.1003954444266072No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)