avigate to the source folder where the files for submission are; Establish an FTP connection using the credentials below: Address: ftp-private.ncbi.nlm.nih.gov Username: subftp Password: 1CVwWYg7 Please do not share these log-in credentials. Do not include these log-in credentials on a public page. These credentials are changed regularly, as per our security policies. Navigate to your account folder: From the command line use the 'cd' command: cd uploads/donohue_ucsc.edu_FGI8lM12 When using a GUI FTP client (eg: Filezilla, NcFTP, Cyberduck, etc.), after you’ve connected to the FTP server, paste your account folder (uploads/donohue_ucsc.edu_FGI8lM12) into the “Remote Site” or “Remote Directory” box on the interface and press “Enter”. Then you will be able to create the data sub-directory for your submission. Until you do this, you will see a message stating "550 /: Permission denied" or "Failed to read the directory listing". We prevent directory listing in the default sign in folder for security reasons. Create a subfolder (required!) with a meaningful name: mkdir new_folder Navigate to the target folder you just created: cd new_folder Copy your files into the target folder: put file_name If you upload your files in your root directory, you will not be able to see them or to select the folder during the submission. Make a new subdirectory for each new submission. Your submission subfolder is a temporary holding area and it will be removed once the whole submission is complete. Do not upload complex directory structures or files that do not contain sequence data. Return back to this page and click the "New submission" button to complete the submission. Please note: it takes at least 10 minutes for uploaded files to become available for selection. Please complete your submission within 30 days of creating a preload folder. If you upload files and do not submit them, they will be automatically deleted 30 days after folder creation.