FastQCFastQC Report
Tue 15 Jan 2019
mjo_001D_S53_L005_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamemjo_001D_S53_L005_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77255243
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATGGGTCGTATGCACAGTGCCGGTAAAGGTATTTCTTCTTCTGCTATT69474118.992801951318695No Hit
GCTGACAAGTCATACATTGAAGGTACTGCTGTTTCTCAAGCTGACGTCAC40122005.193433926549167No Hit
CCACTGAATTAACCGTTCAATCCGAGAGAGCTTTCCAAAAGGTATGAATT36498704.72443015938737No Hit
GCTATTCTAAGTGCCTCCGCTCTAGCTGGTATGTACACATTCTCCTTTTT36296374.698240351143546No Hit
AGGGACATTAAGCAGGATGCTGTAAGAGTATGTTAATACGTGAAATGAGA31301764.051732773657834No Hit
CCACTGAATTAACCGTTCAATCCGAGAGAGCTTTCCAAAAGCAACCACAC28744643.7207364683326407No Hit
CAAGTCTTTTAAGGAAAAACAGTGCGGAAAAATGGCTCACTTTAAAGAAT26310853.405704128068046No Hit
GTTGGCTGCTAACACTCACTTAGGTGCTAGAAACGTTCAAGTTCACCAAG25820333.3422107027739205No Hit
AAATGGGTCGTATGCACAGTGCCGTATGTTTATTAACACCATAGCGAGAT22351682.893224994451186No Hit
GCTATTCTAAGTGCCTCCGCTCTAGCTGCTAACTCAACTACTTCTATTCC20030032.592708173864653No Hit
GAAAGCAACAAGACAACTAAGACTAAGCAACAATGCCAAGAGCTCCAAGA18681902.41820480714817No Hit
CGGTGCTCAAGGTACTAAGTTTAGAATCTCATTAGGTCTACCAGTCGGTG14761761.910777757828035No Hit
CAATAACAATTAAGAATGGCTAACGGTACGTGAAAGGGGTGATATCCTGT14294431.850286070551872No Hit
GTCAAGATCTCGAGACTAGCAATAACAAAATGGGTAGAGTTAGAACCAAG13763381.781546399381593No Hit
GTTGCTACTGATGTGGCCGCCAGAGGTATCGGTATGTTAAAATTTTCTCC13214021.7104366625317584No Hit
AAGAAACCATTAGATCAATAAGCAATGGGTAGAGTTATTCGTAACCAAAG12300231.592154722754545No Hit
GTTGGCTGCTAACACTCACTTAGGTGCTAGAAACGTTCAAGTATGTTATA11717001.5166608174412188No Hit
CCGTAGAATAGGTACAGTGAGACAGTATATTCGAAATGGTATGTTTGAGA9409441.2179678212907827No Hit
CAAGTCTTTTAAGGAAAAACAGTGCGGAAAAAGTGAGTAAATACCTACTA8732711.1303711775264238No Hit
CCGCTGGTGCTAATGTCGACAACGTATGTACAAAAATGCAACCTAAAACG7573590.9803334642284408No Hit
GAAAGCAACAAGACAACTAAGACTAAGCAACAATGCCAAGTACGTATTAG6996210.9055967890748852No Hit
CAATTAATCAATATACGCAGAGATGGCCCCAGTTAAATCCCAAGAATCTA6694020.8664809972832523No Hit
AAAACACACCAGATAATTAGTGCATATATATTAGATCAATATGGTATGTG5659470.7325677559515281No Hit
TTTCTCTGGCTTCCCAGTTACTGGTATGTTATAATAGACAAAATTTTTGG5589750.7235431257396989No Hit
GTCAAGATCTCGAGACTAGCAATAACAAAATGGTATGTTAATATGGACTA5504280.7124797989438724No Hit
AAAACACACCAGATAATTAGTGCATATATATTAGATCAATATGGTTTTAG5327810.6896373363293932No Hit
CGGTGCTCAAGGTACTAAGTTTAGAATCTCAGTATGTTAAAATTTTTATT5296460.6855793593193409No Hit
AAGAAACCATTAGATCAATAAGCAATGGGTATGTCAGCACCGTGATTTAA3887530.5032059765833627No Hit
CGAACCCATTTCTTTGTCCACCACTTTCAAACAATCTTCTCCAGCTAACC3680930.4764634550434331No Hit
CCGTAGAATAGGTACAGTGAGACAGTATATTCGAAATGGCCGGTGTTAAA3402430.44041412179623846No Hit
AGCTGTTCAGGTTAGATGCAGGCTACCAACAATACGACTGGGGTAAAATC3274310.42383013409199943No Hit
GTTGCTACTGATGTGGCCGCCAGAGGTATCGATGTCAAAGGTATCAATTA3237650.41908482509077083No Hit
GAAAACTAATACAGCAACAGAAATACAAAAGTATACAACATGCCAAGTAC3181740.411847775820212No Hit
CATGTCAGACTACGAGGAGGCGTTTAACGACGGTAATGAGAACTTCGAAG3119340.4037706541159931No Hit
GCTCCATTTAGGCAGGACAGTATTTTGGTACGTATTTAGTTCATCAATGG3064840.3967161167300969No Hit
CAATTAATCAATATACGCAGAGATGGTCAGTATAACATGATTTTATAACT2885200.3734633259777592No Hit
ATTTACTGAATTAACAATGGATTCTGAGGTTGCTGCTTTGGTTATTGATA2621070.339274060661488No Hit
CATGTCAGACTACGAGGAGGCGTATGTGCATTCATATTGGAGAAATTGCG2424690.3138544266827301No Hit
CCGCTGGTGCTAATGTCGACAACGTCTGGGCTGATGTTTACGCTAAGGCT2383790.3085602876169841No Hit
GCTGACAAGTCATACATTGAAGGGTATGTTCCGATTTAGTTTACTTTATA2309940.29900106585646236No Hit
CTAGAGTTAGAAGCCCCAATTAAAGTATGTTAATGTTGAACTTCGCAGTC2244150.2904851389827354No Hit
GGACACGATGAAGTTGGATATAAAGGTATGTTACCAGAGCGCGTTTTGCC2224280.2879131452605747No Hit
CAGCATCTCATAATATGTCTGCAACGTATGTACACTAATATCTGAAACGT2124560.2750052834601789No Hit
CGATAAAGAACCAAATAGGACTAAAAATGGCCGGCGTTAAAGCTTACGAA2107930.2728526787495834No Hit
TGAAAAAGGGCGAAGTCAGTTTAGGTACCTCAATGTATGTATATAAGAAT1826240.23639042854347114No Hit
ATTTACTGAATTAACAATGGATTCTGGTATGTTCTAGCGCTTGCACCATC1586680.20538152989823616No Hit
TCCATGCCAGAAGGAGGCCTGCGCCATCCAAGGTTTGTAATATTAACTTC1553750.2011190360245194No Hit
CGATAAAGAACCAAATAGGACTAAAAATGGTATGTAGAGATGAATATACA1477660.1912698663053846No Hit
CAATAACAATTAAGAATGGCTAACGACCTTGTTCAAGCTCGCGATAACTC1386120.17942083231813793No Hit
GCCCGACCTTTGTGTTTCGTACAAGAAAATTGCGCCACCAAAAGGACTAT1288690.16680939052900268No Hit
TGGTACCTACCTGGGTTGCAGCAGCTTGGGTCAAACATTTGTCAAAGGAA1283790.16617512936953677No Hit
CGTATTGCTAAAGAACTAAGTGATCTAGAAAGGTATGTCTAAAGTTATGG1179930.1527313816099187No Hit
GGACACGATGAAGTTGGATATAAAGAAAACTTTTAGTAATAGATCCGATC1148950.14872129778945878No Hit
AACAATAGCAATACGGACTAAAATGGTATGTTGGTGTGTGTGTGTGCTGC1141630.14777378928184848No Hit
GCCCTAAAACACAATGGTTACAGTATGTTGTTGCTTTTATTTACTTTTTC1137740.14727026358586434No Hit
AGTAATAAGCTCTGATCGTTTTGAAAAAGTATGTTTCTTTCCATTGGACG1066130.1380009897839555No Hit
CAGCATCTCATAATATGTCTGCAACTGCTAAACACGACAGTAACGCTAGC1053960.13642569216952694No Hit
TTTCTCTGGCTTCCCAGTTACTGAAGACGGTAAGTGTCCAGGTAAGTTAG1052520.13623929705327573No Hit
TCACTCTCAAATGAAACAATTCGATACCGTATGTAAGATGGTTTTATTGG976870.1264470813974399No Hit
TGTCAGACCCTGTAGAGTTATTGAAAAGAGTATGTAGTAGGGAAATATAT955110.1236304440851995No Hit
AGAGCATCCAGACCAAAACGTATCTCTAACAGAAAAATCAGCCTCATTAA864310.11187719647713747No Hit
GCCCGACCTTTGTGTTTCGTATGTTTTTCACCGACAATTTTCTTTCAAAT848020.10976860172454572No Hit
CCAAAACACAATGAATATTGTCAAATTACAAGTATGTATGCCTTTCTCGA829540.10737653106598863No Hit
GCCAAGGAATTAAGTGATTTAGGGAGGTATGTTAAAAATAAAATGAATGA829230.10733640433957344No Hit
AATGGACCCACATAATCCAATTGGTATGTTGTTGATATTTTTAGTGTTTG828420.10723155708668214No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)