FastQCFastQC Report
Tue 15 Jan 2019
mjo_001C_S52_L005_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamemjo_001C_S52_L005_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83068249
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATGGGTCGTATGCACAGTGCCGGTAAAGGTATTTCTTCTTCTGCTATT887152810.679806191653324No Hit
GCTGACAAGTCATACATTGAAGGTACTGCTGTTTCTCAAGCTGACGTCAC57910636.971451872062453No Hit
GCTATTCTAAGTGCCTCCGCTCTAGCTGCTAACTCAACTACTTCTATTCC47371455.702714402948351No Hit
CCACTGAATTAACCGTTCAATCCGAGAGAGCTTTCCAAAAGCAACCACAC39130374.7106289672724415No Hit
GTTGGCTGCTAACACTCACTTAGGTGCTAGAAACGTTCAAGTTCACCAAG35216554.23947181070351No Hit
CAAGTCTTTTAAGGAAAAACAGTGCGGAAAAATGGCTCACTTTAAAGAAT33645724.050370677730308No Hit
AGGGACATTAAGCAGGATGCTGTAAGAGTATGTTAATACGTGAAATGAGA30331223.6513614245076957No Hit
GAAAGCAACAAGACAACTAAGACTAAGCAACAATGCCAAGAGCTCCAAGA24066242.8971647157267033No Hit
AAATGGGTCGTATGCACAGTGCCGTATGTTTATTAACACCATAGCGAGAT18885012.2734330177105333No Hit
CGGTGCTCAAGGTACTAAGTTTAGAATCTCATTAGGTCTACCAGTCGGTG17077162.0557987203991743No Hit
GCTATTCTAAGTGCCTCCGCTCTAGCTGGTATGTACACATTCTCCTTTTT16107411.9390573647459453No Hit
TGGTACCTACCTGGGTTGCAGCAGCTTGGGTCAAACATTTGTCAAAGGAA15429411.8574377317138346No Hit
GTCAAGATCTCGAGACTAGCAATAACAAAATGGGTAGAGTTAGAACCAAG14470441.741994104149228No Hit
AAGAAACCATTAGATCAATAAGCAATGGGTAGAGTTATTCGTAACCAAAG13382121.6109789433505453No Hit
CAATAACAATTAAGAATGGCTAACGGTACGTGAAAGGGGTGATATCCTGT12366241.4886843226947037No Hit
GTTGCTACTGATGTGGCCGCCAGAGGTATCGGTATGTTAAAATTTTCTCC11937531.4370749526693407No Hit
GTTGCTACTGATGTGGCCGCCAGAGGTATCGATGTCAAAGGTATCAATTA9076811.0926930697672466No Hit
CAATTAATCAATATACGCAGAGATGGCCCCAGTTAAATCCCAAGAATCTA8196750.9867488599645335No Hit
AAAACACACCAGATAATTAGTGCATATATATTAGATCAATATGGTTTTAG6803530.8190289408893162No Hit
CATGTCAGACTACGAGGAGGCGTTTAACGACGGTAATGAGAACTTCGAAG6096580.7339242217564981No Hit
CAAGTCTTTTAAGGAAAAACAGTGCGGAAAAAGTGAGTAAATACCTACTA5597610.6738567463965685No Hit
CCGTAGAATAGGTACAGTGAGACAGTATATTCGAAATGGCCGGTGTTAAA5166090.6219091003109984No Hit
AGCTGTTCAGGTTAGATGCAGGCTACCAACAATACGACTGGGGTAAAATC4968970.5981792152618987No Hit
CCACTGAATTAACCGTTCAATCCGAGAGAGCTTTCCAAAAGGTATGAATT4828640.581285877351285No Hit
CAGCATCTCATAATATGTCTGCAACTGCTAAACACGACAGTAACGCTAGC4548140.5475184628003895No Hit
CGAACCCATTTCTTTGTCCACCACTTTCAAACAATCTTCTCCAGCTAACC3773410.45425418802315193No Hit
ATTTACTGAATTAACAATGGATTCTGAGGTTGCTGCTTTGGTTATTGATA3584980.4315704307189622No Hit
CCGCTGGTGCTAATGTCGACAACGTATGTACAAAAATGCAACCTAAAACG3532930.4253044987140634No Hit
GAAAGCAACAAGACAACTAAGACTAAGCAACAATGCCAAGTACGTATTAG3515490.42320502024786866No Hit
GTTGGCTGCTAACACTCACTTAGGTGCTAGAAACGTTCAAGTATGTTATA3503600.42177366709631736No Hit
GAAAACTAATACAGCAACAGAAATACAAAAGTATACAACATGCCAAGTAC3293940.3965341799849423No Hit
GGACACGATGAAGTTGGATATAAAGAAAACTTTTAGTAATAGATCCGATC2701180.3251759887222373No Hit
CCGCTGGTGCTAATGTCGACAACGTCTGGGCTGATGTTTACGCTAAGGCT2517290.3030387699637198No Hit
CAATAACAATTAAGAATGGCTAACGACCTTGTTCAAGCTCGCGATAACTC2507300.3018361443973617No Hit
CGATAAAGAACCAAATAGGACTAAAAATGGCCGGCGTTAAAGCTTACGAA2392130.28797164124646474No Hit
TTTCTCTGGCTTCCCAGTTACTGGTATGTTATAATAGACAAAATTTTTGG2372240.2855772245783103No Hit
TTTCTCTGGCTTCCCAGTTACTGAAGACGGTAAGTGTCCAGGTAAGTTAG2321760.2795002937885449No Hit
GCTCCATTTAGGCAGGACAGTATTTTGATAATATACCCCAGATCTCAAAC2312680.27840721669720037No Hit
GCCCGACCTTTGTGTTTCGTACAAGAAAATTGCGCCACCAAAAGGACTAT2233100.2688271423657913No Hit
CATGTCAGACTACGAGGAGGCGTATGTGCATTCATATTGGAGAAATTGCG1864690.22447686359682384No Hit
TGAAAAAGGGCGAAGTCAGTTTAGGTACCTCAATTATGGCCGTGACATTT1715330.2064964677418444No Hit
CCGTAGAATAGGTACAGTGAGACAGTATATTCGAAATGGTATGTTTGAGA1690470.20350374786400036No Hit
CGGTGCTCAAGGTACTAAGTTTAGAATCTCAGTATGTTAAAATTTTTATT1679090.2021337900116325No Hit
GCCCTAAAACACAATGGTTACACAACAAGAAAAGGAATTTTTAGAATCCT1671820.20125860604091944No Hit
CAATTAATCAATATACGCAGAGATGGTCAGTATAACATGATTTTATAACT1627070.19587146949492096No Hit
AGTAATAAGCTCTGATCGTTTTGAAAAATGAACTTCAATACACCTCAACA1446380.17411947614304474No Hit
GCTCCATTTAGGCAGGACAGTATTTTGGTACGTATTTAGTTCATCAATGG1407560.16944621042872832No Hit
GTCAAGATCTCGAGACTAGCAATAACAAAATGGTATGTTAATATGGACTA1345010.161916257558288No Hit
AATGGACCCACATAATCCAATTGTCCTTGATCAGGGTACTGGTTTCGTCA1295110.15590914887347632No Hit
TGTGTCTACAGCGTCTTCAGGAAGAAAGAAAAAAATGGAGAAAGGATCAT1270820.15298504727119022No Hit
TCCATGCCAGAAGGAGGCCTGCGCCATCCAAGGTTTGTAATATTAACTTC1228870.14793498295600283No Hit
AAGAAACCATTAGATCAATAAGCAATGGGTATGTCAGCACCGTGATTTAA1148270.13823211802622684No Hit
AAAACACACCAGATAATTAGTGCATATATATTAGATCAATATGGTATGTG1130900.13614106636580242No Hit
GAAAACTAATACAGCAACAGAAATACAAAAGTATACAACATGCCAAGAGC1118690.134671190673587No Hit
CCACTGAATTAACCGTTCAATCCGAGAGAGCTCTCCAAAAGCAACCACAC1091680.13141964747565585No Hit
TGAAAAAGGGCGAAGTCAGTTTAGGTACCTCAATGTATGTATATAAGAAT973590.11720362614119867No Hit
AGAGCATCCAGACCAAAACGTATCTCTAACAGAAAAATCAGCCTCATTAA947330.11404237014794906No Hit
TGTCAGACCCTGTAGAGTTATTGAAAAGAGCTGAGAAGAAGGGTGTTCCT942880.1135066660668386No Hit
AATACATTGGACAGAAATTATGGACGATGGACGGAAAAAAGAATTGCATG937880.11290475136896169No Hit
GCTGACAAGTCATACATTGAAGGGTATGTTCCGATTTAGTTTACTTTATA929660.11191520360565202No Hit
AGGGACATTAAGCAGGATGCTGTAAGAGAATTTTTTGCATCTCAAGTAGG928960.11183093554794925No Hit
GTTGGGATATTTTTGGTTGGTTCAGAGATGTGTTGGCTTCCCTTGGTCTG877590.10564686394196174No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)