FastQCFastQC Report
Tue 15 Jan 2019
mjo_001B_S51_L005_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamemjo_001B_S51_L005_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106240399
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATGGGTCGTATGCACAGTGCCGGTAAAGGTATTTCTTCTTCTGCTATT1296677712.20512829587547No Hit
CAAGTCTTTTAAGGAAAAACAGTGCGGAAAAATGGCTCACTTTAAAGAAT73619686.929537228112255No Hit
GCTGACAAGTCATACATTGAAGGTACTGCTGTTTCTCAAGCTGACGTCAC68403506.438558273863411No Hit
CCACTGAATTAACCGTTCAATCCGAGAGAGCTTTCCAAAAGCAACCACAC65555946.1705284069951585No Hit
GTTGGCTGCTAACACTCACTTAGGTGCTAGAAACGTTCAAGTTCACCAAG47389124.460555536881973No Hit
GAAAGCAACAAGACAACTAAGACTAAGCAACAATGCCAAGAGCTCCAAGA45193074.253849799641659No Hit
GCTATTCTAAGTGCCTCCGCTCTAGCTGCTAACTCAACTACTTCTATTCC43210904.067275763902205No Hit
AGGGACATTAAGCAGGATGCTGTAAGAGTATGTTAATACGTGAAATGAGA39133173.6834547279891146No Hit
CGGTGCTCAAGGTACTAAGTTTAGAATCTCATTAGGTCTACCAGTCGGTG31892143.001884433811285No Hit
GTCAAGATCTCGAGACTAGCAATAACAAAATGGGTAGAGTTAGAACCAAG30465012.867554177766219No Hit
CAATTAATCAATATACGCAGAGATGGCCCCAGTTAAATCCCAAGAATCTA26452722.489892757274001No Hit
AAGAAACCATTAGATCAATAAGCAATGGGTAGAGTTATTCGTAACCAAAG22434592.1116816400510694No Hit
AAAACACACCAGATAATTAGTGCATATATATTAGATCAATATGGTTTTAG15300971.4402214359153527No Hit
GCTATTCTAAGTGCCTCCGCTCTAGCTGGTATGTACACATTCTCCTTTTT11201311.0543362134775116No Hit
CATGTCAGACTACGAGGAGGCGTTTAACGACGGTAATGAGAACTTCGAAG10576830.9955563137521726No Hit
CCGTAGAATAGGTACAGTGAGACAGTATATTCGAAATGGCCGGTGTTAAA8824650.8306303518306628No Hit
AGCTGTTCAGGTTAGATGCAGGCTACCAACAATACGACTGGGGTAAAATC6875340.6471493014629962No Hit
CGATAAAGAACCAAATAGGACTAAAAATGGCCGGCGTTAAAGCTTACGAA5850000.5506379922387151No Hit
CCGCTGGTGCTAATGTCGACAACGTCTGGGCTGATGTTTACGCTAAGGCT5639090.5307858454108404No Hit
AAATGGGTCGTATGCACAGTGCCGTATGTTTATTAACACCATAGCGAGAT5549860.5223869688215308No Hit
TCCATGCCAGAAGGAGGCCTGCGCCATCCAAGGTTTGTAATATTAACTTC5532420.5207454087215919No Hit
ATTTACTGAATTAACAATGGATTCTGAGGTTGCTGCTTTGGTTATTGATA4961840.4670389086170507No Hit
CAATAACAATTAAGAATGGCTAACGGTACGTGAAAGGGGTGATATCCTGT4396920.41386516253576944No Hit
CAGCATCTCATAATATGTCTGCAACTGCTAAACACGACAGTAACGCTAGC4238820.3989838178224463No Hit
GTTGCTACTGATGTGGCCGCCAGAGGTATCGATGTCAAAGGTATCAATTA4148660.3904974039112937No Hit
CAATAACAATTAAGAATGGCTAACGACCTTGTTCAAGCTCGCGATAACTC3667400.3451982517497887No Hit
GCCCGACCTTTGTGTTTCGTACAAGAAAATTGCGCCACCAAAAGGACTAT3581350.337098696325491No Hit
GCCCTAAAACACAATGGTTACACAACAAGAAAAGGAATTTTTAGAATCCT3340910.3144670042137172No Hit
GCTCCATTTAGGCAGGACAGTATTTTGATAATATACCCCAGATCTCAAAC3231110.30413195266708287No Hit
TGGTACCTACCTGGGTTGCAGCAGCTTGGGTCAAACATTTGTCAAAGGAA3193810.30062104717810784No Hit
TGAAAAAGGGCGAAGTCAGTTTAGGTACCTCAATTATGGCCGTGACATTT3184320.2997277899906984No Hit
CGAACCCATTTCTTTGTCCACCACTTTCAAACAATCTTCTCCAGCTAACC2857780.2689918361470009No Hit
GGACACGATGAAGTTGGATATAAAGAAAACTTTTAGTAATAGATCCGATC2855720.2687979362728109No Hit
TCACTCTCAAATGAAACAATTCGATACCGTATGTAAGATGGTTTTATTGG2800370.26358805373086No Hit
TCACTCTCAAATGAAACAATTCGATACCAAGTACTCTGGTAATAGAATTT2770770.26080191961628457No Hit
TGTGTCTACAGCGTCTTCAGGAAGAAAGAAAAAAATGGAGAAAGGATCAT2496490.23498499850325297No Hit
CATGTCAGACTACGAGGAGGCGTATGTGCATTCATATTGGAGAAATTGCG2445090.23014691426375386No Hit
GAAAACTAATACAGCAACAGAAATACAAAAGTATACAACATGCCAAGTAC2431520.22886962237406508No Hit
TTTCTCTGGCTTCCCAGTTACTGAAGACGGTAAGTGTCCAGGTAAGTTAG2009680.18916344619526515No Hit
GTTGGGATATTTTTGGTTGGTTCAGAGATGTGTTGGCTTCCCTTGGTCTG1965060.1849635372698478No Hit
AGGGACATTAAGCAGGATGCTGTAAGAGAATTTTTTGCATCTCAAGTAGG1934840.18211904494071035No Hit
AGAGCATCCAGACCAAAACGTATCTCTAACAGAAAAATCAGCCTCATTAA1922460.1809537631725197No Hit
CCACTGAATTAACCGTTCAATCCGAGAGAGCTTTCCAAAAGGTATGAATT1901120.17894511107775488No Hit
TGAAAAAGGGCGAAGTCAGTTTAGGTACCTCAATGTATGTATATAAGAAT1823480.1716371565961457No Hit
GCTCCATTTAGGCAGGACAGTATTTTGGTACGTATTTAGTTCATCAATGG1806630.17005113092619314No Hit
CAAGTCTTTTAAGGAAAAACAGTGCGGAAAAAGTGAGTAAATACCTACTA1719510.16185086051869968No Hit
TTTCTCTGGCTTCCCAGTTACTGGTATGTTATAATAGACAAAATTTTTGG1705570.1605387419525787No Hit
GTTGCTACTGATGTGGCCGCCAGAGGTATCGGTATGTTAAAATTTTCTCC1677330.15788061940542975No Hit
AATGGACCCACATAATCCAATTGTCCTTGATCAGGGTACTGGTTTCGTCA1596980.15031758305049286No Hit
TGTCAGACCCTGTAGAGTTATTGAAAAGAGCTGAGAAGAAGGGTGTTCCT1528300.14385299889545783No Hit
GAAAGCAACAAGACAACTAAGACTAAGCAACAATGCCAAGTACGTATTAG1442390.1357666211325129No Hit
CTTGATGCTGCCTGTATTTTTTGCAAGATTATTAAAAGCGAAATTCCATC1319380.12418816311109676No Hit
CGGTGCTCAAGGTACTAAGTTTAGAATCTCAGTATGTTAAAATTTTTATT1285980.12104434961694752No Hit
AAACGGGAAAAGTCACTGGATCACACATTAGAACTAAAGATACCGTTTGA1248560.1175221489896701No Hit
CTAGAGTTAGAAGCCCCAATTAAAATATGTGGTGACATTCATGGGCAATA1225280.1153308921590176No Hit
CCACTGAATTAACCGTTCAATCCGAGAGAGCTCTCCAAAAGCAACCACAC1224690.11527535772903111No Hit
AGTAATAAGCTCTGATCGTTTTGAAAAATGAACTTCAATACACCTCAACA1127680.1061441796731204No Hit
CAGCATCTCATAATATGTCTGCAACGTATGTACACTAATATCTGAAACGT1111780.10464757384806134No Hit
TCTGCCTCCAAACAAAGCGTACGTTAATTTTACAAGTTTTGCCTTTAGAA1098150.10336463438922137No Hit
CCGCTGGTGCTAATGTCGACAACGTATGTACAAAAATGCAACCTAAAACG1078440.10150940792306325No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)