FastQCFastQC Report
Tue 15 Jan 2019
mjo_001A_S50_L005_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamemjo_001A_S50_L005_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80898093
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATGGGTCGTATGCACAGTGCCGGTAAAGGTATTTCTTCTTCTGCTATT1055672713.049413908928607No Hit
GCTGACAAGTCATACATTGAAGGTACTGCTGTTTCTCAAGCTGACGTCAC49475176.115739959407943No Hit
CAAGTCTTTTAAGGAAAAACAGTGCGGAAAAATGGCTCACTTTAAAGAAT46671515.769173075563104No Hit
CCACTGAATTAACCGTTCAATCCGAGAGAGCTTTCCAAAAGCAACCACAC38887784.807008244310531No Hit
GAAAGCAACAAGACAACTAAGACTAAGCAACAATGCCAAGAGCTCCAAGA31197773.856428358577006No Hit
GTTGGCTGCTAACACTCACTTAGGTGCTAGAAACGTTCAAGTTCACCAAG30674373.791729676495588No Hit
GTCAAGATCTCGAGACTAGCAATAACAAAATGGGTAGAGTTAGAACCAAG28662543.54304272660667No Hit
CAATTAATCAATATACGCAGAGATGGCCCCAGTTAAATCCCAAGAATCTA25462483.147475923814422No Hit
GCTATTCTAAGTGCCTCCGCTCTAGCTGCTAACTCAACTACTTCTATTCC25174423.1118681623310946No Hit
AAGAAACCATTAGATCAATAAGCAATGGGTAGAGTTATTCGTAACCAAAG19963902.4677837585120823No Hit
CGGTGCTCAAGGTACTAAGTTTAGAATCTCATTAGGTCTACCAGTCGGTG19265672.381473936598234No Hit
AGGGACATTAAGCAGGATGCTGTAAGAGTATGTTAATACGTGAAATGAGA18607282.300088829040754No Hit
AAAACACACCAGATAATTAGTGCATATATATTAGATCAATATGGTTTTAG17321022.1410912615702817No Hit
CATGTCAGACTACGAGGAGGCGTTTAACGACGGTAATGAGAACTTCGAAG7315120.9042388675342445No Hit
AAATGGGTCGTATGCACAGTGCCGTATGTTTATTAACACCATAGCGAGAT7203240.89040912249934No Hit
ATTTACTGAATTAACAATGGATTCTGAGGTTGCTGCTTTGGTTATTGATA6367720.7871285668007032No Hit
CCGCTGGTGCTAATGTCGACAACGTCTGGGCTGATGTTTACGCTAAGGCT6336770.7833027658637144No Hit
GCTATTCTAAGTGCCTCCGCTCTAGCTGGTATGTACACATTCTCCTTTTT6246970.7722023805925808No Hit
CCGTAGAATAGGTACAGTGAGACAGTATATTCGAAATGGCCGGTGTTAAA5532880.6839320674716028No Hit
CAATAACAATTAAGAATGGCTAACGGTACGTGAAAGGGGTGATATCCTGT5500620.6799443344109484No Hit
CGATAAAGAACCAAATAGGACTAAAAATGGCCGGCGTTAAAGCTTACGAA5175990.6398160708188758No Hit
AGCTGTTCAGGTTAGATGCAGGCTACCAACAATACGACTGGGGTAAAATC4503470.5566843213473524No Hit
GTTGCTACTGATGTGGCCGCCAGAGGTATCGATGTCAAAGGTATCAATTA4033070.49853709159744963No Hit
CAGCATCTCATAATATGTCTGCAACTGCTAAACACGACAGTAACGCTAGC3372050.416826883669557No Hit
TCCATGCCAGAAGGAGGCCTGCGCCATCCAAGGTTTGTAATATTAACTTC3182440.39338875392279027No Hit
CCACTGAATTAACCGTTCAATCCGAGAGAGCTTTCCAAAAGGTATGAATT2655910.32830316531688825No Hit
CAAGTCTTTTAAGGAAAAACAGTGCGGAAAAAGTGAGTAAATACCTACTA2623010.324236320379023No Hit
GCCCTAAAACACAATGGTTACACAACAAGAAAAGGAATTTTTAGAATCCT2608060.3223883163723031No Hit
CAATAACAATTAAGAATGGCTAACGACCTTGTTCAAGCTCGCGATAACTC2569950.31767745130901914No Hit
CGAACCCATTTCTTTGTCCACCACTTTCAAACAATCTTCTCCAGCTAACC2388110.2951997891965142No Hit
TTTCTCTGGCTTCCCAGTTACTGAAGACGGTAAGTGTCCAGGTAAGTTAG2034240.2514571017143754No Hit
GGACACGATGAAGTTGGATATAAAGAAAACTTTTAGTAATAGATCCGATC2028040.2506907054038962No Hit
GAAAGCAACAAGACAACTAAGACTAAGCAACAATGCCAAGTACGTATTAG1971210.24366581793219774No Hit
GCTCCATTTAGGCAGGACAGTATTTTGATAATATACCCCAGATCTCAAAC1935050.23919599686979026No Hit
GAAAACTAATACAGCAACAGAAATACAAAAGTATACAACATGCCAAGTAC1919270.23724539464731265No Hit
GCCCGACCTTTGTGTTTCGTACAAGAAAATTGCGCCACCAAAAGGACTAT1904680.23544189107152377No Hit
TGAAAAAGGGCGAAGTCAGTTTAGGTACCTCAATTATGGCCGTGACATTT1797370.2221770542848272No Hit
GTTGGGATATTTTTGGTTGGTTCAGAGATGTGTTGGCTTCCCTTGGTCTG1760320.21759721826817352No Hit
CGTATTGCTAAAGAACTAAGTGATCTAGAAAGAGATCCACCTACTTCATG1726720.21344384471460903No Hit
TCACTCTCAAATGAAACAATTCGATACCAAGTACTCTGGTAATAGAATTT1651770.20417910221938113No Hit
CAATTAATCAATATACGCAGAGATGGTCAGTATAACATGATTTTATAACT1650010.20396154455705157No Hit
CCGCTGGTGCTAATGTCGACAACGTATGTACAAAAATGCAACCTAAAACG1635540.2021728744582397No Hit
TGGTACCTACCTGGGTTGCAGCAGCTTGGGTCAAACATTTGTCAAAGGAA1332950.1647690261376124No Hit
TTTCTCTGGCTTCCCAGTTACTGGTATGTTATAATAGACAAAATTTTTGG1282130.15848704863784613No Hit
AATGGACCCACATAATCCAATTGTCCTTGATCAGGGTACTGGTTTCGTCA1265890.15647958475362328No Hit
AGGGACATTAAGCAGGATGCTGTAAGAGAATTTTTTGCATCTCAAGTAGG1241020.15340534665013672No Hit
GTTGGCTGCTAACACTCACTTAGGTGCTAGAAACGTTCAAGTATGTTATA1229820.15202088879894857No Hit
CTTGATGCTGCCTGTATTTTTTGCAAGATTATTAAAAGCGAAATTCCATC1216090.15032369180816166No Hit
CTAGAGTTAGAAGCCCCAATTAAAATATGTGGTGACATTCATGGGCAATA1207400.14924950085040942No Hit
TGTCAGACCCTGTAGAGTTATTGAAAAGAGCTGAGAAGAAGGGTGTTCCT1105310.13662992031221305No Hit
CCGTAGAATAGGTACAGTGAGACAGTATATTCGAAATGGTATGTTTGAGA1077890.1332404708229649No Hit
TGTGTCTACAGCGTCTTCAGGAAGAAAGAAAAAAATGGAGAAAGGATCAT1027780.12704625806197928No Hit
AAAACACACCAGATAATTAGTGCATATATATTAGATCAATATGGTATGTG1016400.12563954999532562No Hit
CGGTGCTCAAGGTACTAAGTTTAGAATCTCAGTATGTTAAAATTTTTATT991460.1225566590302691No Hit
GTTGCTACTGATGTGGCCGCCAGAGGTATCGGTATGTTAAAATTTTCTCC980590.12121299324076774No Hit
CATGTCAGACTACGAGGAGGCGTATGTGCATTCATATTGGAGAAATTGCG913390.11290624613363878No Hit
AGAGCATCCAGACCAAAACGTATCTCTAACAGAAAAATCAGCCTCATTAA906830.11209534939222857No Hit
GCTGACAAGTCATACATTGAAGGGTATGTTCCGATTTAGTTTACTTTATA840350.1038776031469617No Hit
GTCAAGATCTCGAGACTAGCAATAACAAAATGGTATGTTAATATGGACTA831250.10275273114287133No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)